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A Szentágothai János Kutatóközpont a PTE korszerű, nemzetközi tudományszervezési és menedzsment normák szerint kialakított új intézménye, amely az élettudományi, élettelen természettudományi, valamint környezettudományi oktatás...

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Microbial biotechnology research group

  • Research concept
  • Members
  • R and D results
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  • Laboratories, instruments
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Popular views classify microbes either as a good or bad guys, however these phenomenon imply very complex processes in reality. It is no exaggeration to say that to understand host-microbe-environment interactions at the level of biological complexity is one of the greatest challenges in biology. Recently genomics, proteomics, high-throughput microarray and next-generation sequencing (NGS) technologies have extended our ability to provide new insight into the molecular basis of these fundamental questions.

As the problem of antimicrobial resistance becomes more widespread, the need for new anti-infective agents is more urgent than ever. As a part of these efforts, massively parallel NGS technologies provide a tremendous ability to typify microbes, not only on the basis of readily observable characteristics, but also upon their genetic (biosynthetic) potential.

In respect of the clinical needs, on the bases of our ongoing microbial whole genome shotgun sequencing (WGS) project, we designed strategy and workflow to isolate new microbial natural products (e.g. antimicrobials) and screening for potential producing microorganisms. It is important to highlight the fact that the lack of genome sequence information in non-model species often limits the downstream scientific activities, such as engineering microbial metabolic pathways. We hope that our structural genomics efforts will form a foundation for the subsequent research steps, like intelligent drug design, identification of new pathogenicity factors and target discovery etc.

State of art genomics approaches provides revolutionary tools with numerous applications, which can address different questions in almost all aspects of biological research. Nevertheless, one of the major challenges biologists are facing today is to understand and be able to interpret the functional complement (transcriptome, proteome) of the genome, which is a dynamic link between genotype and complex phenotype. Based on the assumption of one technology being able to standalone does not suffice for gaining a comprehensive understanding of any complex biological process. Therefore we intend to use complementary approaches i.e. transcriptomics (RNA-seq, microarray) and proteomics (2D PAGE) for developing molecular diagnostic methods to identify drug-, toxicant- and disease-related biomarkers.

Due to the broad range of microbial model systems formally used, and extensive research experience in the fields of traditional and molecular-microbiology, the Microbial Biotechnology Group is ready to face challenges in health, industry and agriculture sectors of biotechnology.

Kitti Csepregi, Andrea Valasek, Ágota Pénzes, Zsuzsanna Tóth, Éva Írisz Kiss, Ildikó Kerepesi, Balázs Horváth, István Nagy and Csaba Fekete (2012): Draft Genome Sequence of an Efficient Antibiotics Producing Industrial Strain (SZMC 14600) of Saccharomonospora azurea. J. Bacteriol. 194, No. 5, in press. IF.: 3.72.

Balázs Horváth, Judit Hunyadkürti, Andrea Vörös, Csaba Fekete, Edit Urbán, Lajos Kemény, and István Nagy (2012): Genome Sequence of Propionibacterium acnes Type II Strain ATCC 11828. J. Bacteriol. 194:202-203. IF.: 3.72.

Juhász, Á., Pénzes, Á., Peteri, Z, A., Pallos, J., P., Seffer, D., Feiszt, P., Pesti, M., Fekete, C., Vágvölgyi, C., gazdag, Z., Papp, G. (2011): Process for producing primycin, primycin conponent(s), precursors and metabolites thereof via fermentation by the use of bacterial species Saccharomonospora azurea. WO/2011/051741, 1-21. International Application No.: PCT/HU2010/000116.

Health, industrial and agricultural sectors of biotechnology. Structural- functional- and, comparative.genomics.

  • Polyphasic taxonomical characterization of microbes
  • Development of nucleic acid based diagnostic tools
  • Improvement of microbial strains and fermentation processes for the overproduction of industrial products
  • De novo whole genome shotgun sequencing
  • NGS-based transcriptome profiling
  • BIOSTAT Q plus new generation Fermentor-Bioreactor system
  • Next-generation and capillary sequencing platforms
  • epMotion 5070 automated pipetting system
  • FluorChem Q system for quantitative Western blot imaging
  • Two-dimensional gel electrophoresis system
  • Microfluidics-based Agilent 2100 Bioanalyzer platform for sizing, quantification and quality control of DNA, RNA, proteins
  • Ultra-fast endpoint, gradient and real-time PCR
  • Denaturing Gradient Gel Electrophoresis (DDGE) to detect single-strand conformation polymorphism (SSCP), heteroduplex analysis (HA), and protein truncation tests (PTT)