About us

A Szentágothai János Kutatóközpont a PTE korszerű, nemzetközi tudományszervezési és menedzsment normák szerint kialakított új intézménye, amely az élettudományi, élettelen természettudományi, valamint környezettudományi oktatás...

Tovább

Bejelentkezés

CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

Login


Neurotrauma Research Group

  • Research concept
  • Members
  • Publications
  • Awarded projects
  • Services
  • Laboratories, instruments
  • Galleries

Our research group investigate different potential diagnostic, therapeutic possibilities and the clinical/functional outcome in case of traumatic brain injury (TBI) applying translational research strategies:

a,         Experimental neurotraumatotlogy:

Investigation of traumatic brain injury (TBI) with the utilization of our two licensed neurotrauma animal models: a) the impact acceleration head injury model – described by Marmarou et al. 1994 – and b) the fluid percussion head injury model – described first (for rats) by Dixon et al. 1987. Quantification of the impact of the injury – in strong collaboration with many other research groups of the Centre – involve histological methods (immunohistochemistry; silver staining methods), functional tests, measurement of protein biomarker levels as well as neuro-imaging (MRI, microCT) techniques. Investigation of histological, blood based biomarker and functional alterations – as a consequence of mild/repetitive mild TBI – represent the main focus of our recent experiments.

b,         Clinical studies in TBI:

Maintenance of the serum biobank with the serum samples from more than 3500 TBI subjects from around 60 sites across Europe as part of the CENTER-TBI (‘Collaborative European NeuroTrauma Effectiveness Research in TBI’) consortium. Protein biomarker assays, analyses as well as publication of the results are in progress in this project.

Further construction and targeted statistical analyses of the ’Pécs severe head injury database’ with the clinical and outcome data of all sTBI subjects treated by the Department of Neurosurgery from the 1st of July 2002 – so far the data of more than 700 consecutive cases were included.



Dr. Endre Czeiter
research fellow
endre.czeiter@gmail.com
Krisztina Amrein
assistant research fellow
tina.amrein84@gmail.com
Bálint Fazekas
PhD student
b.fazekas91@gmail.com
József Nyirádi
laboratory assistant
nyiradi.jozsef@pte.hu
+36-72-501-500/29242
Anna Erdélyi
laboratory assistant
erdelyi.anna@pte.hu
+36-72-501-500/29242

Czeiter E, Amrein K, Gravesteijn BY, Lecky F, Menon DK, Mondello S, Newcombe VFJ, Richter S, Steyerberg EW, Vyvere TV, Verheyden J, Xu H, Yang Z, Maas AIR, Wang KKW, Büki A; CENTER-TBI Participants and Investigators. Blood biomarkers on admission in acute traumatic brain injury: Relations to severity, CT findings and care path in the CENTER-TBI study. EBioMedicine. 2020 Jun;56:102785. doi: 10.1016/j.ebiom.2020.102785.

Mondello S, Guedes VA, Lai C, Czeiter E, Amrein K, Kobeissy F, Mechref Y, Jeromin A, Mithani S, Martin C, Wagner CL, Czigler A, Tóth L, Fazekas B, Buki A, Gill J. Circulating Brain Injury Exosomal Proteins following Moderate-To-Severe Traumatic Brain Injury: Temporal Profile, Outcome Prediction and Therapy Implications. Cells. 2020 Apr 15;9(4):977. doi: 10.3390/cells9040977.

Tadepalli SA, Bali ZK, Bruszt N, Nagy LV, Amrein K, Fazekas B, Büki A, Czeiter E, Hernádi I. Long-term cognitive impairment without diffuse axonal injury following repetitive mild traumatic brain injury in rats. Behav Brain Res. 2020 Jan 27;378:112268. doi: 10.1016/j.bbr.2019.112268.

Steyerberg EW, Wiegers E, Sewalt C, Buki A, Citerio G, De Keyser V, Ercole A, Kunzmann K, Lanyon L, Lecky F, Lingsma H, Manley G, Nelson D, Peul W, Stocchetti N, von Steinbüchel N, Vande Vyvere T, Verheyden J, Wilson L, Maas AIR, Menon DK; CENTER-TBI Participants and Investigators. Case-mix, care pathways, and outcomes in patients with traumatic brain injury in CENTER-TBI: a European prospective, multicentre, longitudinal, cohort study. Lancet Neurol. 2019 Oct;18(10):923-934. doi: 10.1016/S1474-4422(19)30232-7.

Czigler A, Toth L, Szarka N, Berta G, Amrein K, Czeiter E, Lendvai-Emmert D, Bodo K, Tarantini S, Koller A, Ungvari Z, Buki A, Toth P. Hypertension Exacerbates Cerebrovascular Oxidative Stress Induced by Mild Traumatic Brain Injury: Protective Effects of the Mitochondria-Targeted Antioxidative Peptide SS-31. J Neurotrauma. 2019 Dec 1;36(23):3309-3315. doi: 10.1089/neu.2019.6439.

Tóth A, Berente Z, Bogner P, Környei B, Balogh B, Czeiter E, Amrein K, Dóczi T, Büki A, Schwarcz A. Cerebral Microbleeds Temporarily Become Less Visible or Invisible in Acute Susceptibility Weighted Magnetic Resonance Imaging: A Rat Study. J Neurotrauma. 2019 May 15;36(10):1670-1677. doi: 10.1089/neu.2018.6004.

Tamás V, Kocsor F, Gyuris P, Kovács N, Czeiter E, Büki A. The Young Male Syndrome-An Analysis of Sex, Age, Risk Taking and Mortality in Patients With Severe Traumatic Brain Injuries. Front Neurol. 2019 Apr 12;10:366. doi: 10.3389/fneur.2019.00366.

Szarka N, Toth L, Czigler A, Kellermayer Z, Ungvari Z, Amrein K, Czeiter E, Bali ZK, Tadepalli SA, Wahr M, Hernadi I, Koller A, Buki A, Toth P. Single Mild Traumatic Brain Injury Induces Persistent Disruption of the Blood-Brain Barrier, Neuroinflammation and Cognitive Decline in Hypertensive Rats. Int J Mol Sci. 2019 Jun 30;20(13):3223. doi: 10.3390/ijms20133223.

Sorinola A, Buki A, Sandor J, Czeiter E. Risk Factors of External Ventricular Drain Infection: Proposing a Model for Future Studies. Front Neurol. 2019 Mar 15;10:226. doi: 10.3389/fneur.2019.00226.

Bazarian JJ, Biberthaler P, Welch RD, Lewis LM, Barzo P, Bogner-Flatz V, Gunnar Brolinson P, Büki A, Chen JY, Christenson RH, Hack D, Huff JS, Johar S, Jordan JD, Leidel BA, Lindner T, Ludington E, Okonkwo DO, Ornato J, Peacock WF, Schmidt K, Tyndall JA, Vossough A, Jagoda AS. Serum GFAP and UCH-L1 for prediction of absence of intracranial injuries on head CT (ALERT-TBI): a multicentre observational study. Lancet Neurol. 2018 Sep;17(9):782-789. doi: 10.1016/S1474-4422(18)30231-X.

Szarka N, Pabbidi MR, Amrein K, Czeiter E, Berta G, Pohoczky K, Helyes Z, Ungvari Z, Koller A, Buki A, Toth P. Traumatic Brain Injury Impairs Myogenic Constriction of Cerebral Arteries: Role of Mitochondria-Derived H2O2 and TRPV4-Dependent Activation of BKca Channels. J Neurotrauma. 2018 Apr 1;35(7):930-939. doi: 10.1089/neu.2017.5056.

Sorinola A, Buki A, Sandor J, Czeiter E. Effectiveness of Traumatic Brain Injury Management Guideline Introduction in Hungary. Turk Neurosurg. 2018;28(3):410-415. doi: 10.5137/1019-5149.JTN.19396-16.1.

Maas AIR, Menon DK, Adelson PD, Andelic N, Bell MJ, Belli A, Bragge P, Brazinova A, Büki A, Chesnut RM, Citerio G, Coburn M, Cooper DJ, Crowder AT, Czeiter E, Czosnyka M, Diaz-Arrastia R, Dreier JP, Duhaime AC, Ercole A, van Essen TA, Feigin VL, Gao G, Giacino J, Gonzalez-Lara LE, Gruen RL, Gupta D, Hartings JA, Hill S, Jiang JY, Ketharanathan N, Kompanje EJO, Lanyon L, Laureys S, Lecky F, Levin H, Lingsma HF, Maegele M, Majdan M, Manley G, Marsteller J, Mascia L, McFadyen C, Mondello S, Newcombe V, Palotie A, Parizel PM, Peul W, Piercy J, Polinder S, Puybasset L, Rasmussen TE, Rossaint R, Smielewski P, Söderberg J, Stanworth SJ, Stein MB, von Steinbüchel N, Stewart W, Steyerberg EW, Stocchetti N, Synnot A, Te Ao B, Tenovuo O, Theadom A, Tibboel D, Videtta W, Wang KKW, Williams WH, Wilson L, Yaffe K; InTBIR Participants and Investigators. Traumatic brain injury: integrated approaches to improve prevention, clinical care, and research. Lancet Neurol. 2017 Dec;16(12):987-1048. doi: 10.1016/S1474-4422(17)30371-X.

Maegele M, Schöchl H, Menovsky T, Maréchal H, Marklund N, Buki A, Stanworth S. Coagulopathy and haemorrhagic progression in traumatic brain injury: advances in mechanisms, diagnosis, and management. Lancet Neurol. 2017 Aug;16(8):630-647. doi: 10.1016/S1474-4422(17)30197-7.

Thelin EP, Zeiler FA, Ercole A, Mondello S, Büki A, Bellander BM, Helmy A, Menon DK, Nelson DW. Serial Sampling of Serum Protein Biomarkers for Monitoring Human Traumatic Brain Injury Dynamics: A Systematic Review. Front Neurol. 2017 Jul 3;8:300. doi: 10.3389/fneur.2017.00300.

Szarka N, Amrein K, Horvath P, Ivic I, Czeiter E, Buki A, Koller A, Toth P. Hypertension-Induced Enhanced Myogenic Constriction of Cerebral Arteries Is Preserved after Traumatic Brain Injury. J Neurotrauma. 2017 Jul 15;34(14):2315-2319. doi: 10.1089/neu.2016.4962.

Tamás V, Kovács N, Büki A. A sportolás és a baleseti agysérülés összefüggései - veszélyek és következmények/Relationship between sport and traumatic brain injury - risks and sequelaes. Ideggyogy Sz. 2017 Mar 30;70(3-4):89-96. Hungarian. doi: 10.18071/isz.70.0089.

Toth P, Szarka N, Farkas E, Ezer E, Czeiter E, Amrein K, Ungvari Z, Hartings JA, Buki A, Koller A. Traumatic brain injury-induced autoregulatory dysfunction and spreading depression-related neurovascular uncoupling: Pathomechanisms, perspectives, and therapeutic implications. Am J Physiol Heart Circ Physiol. 2016 Nov 1;311(5):H1118-H1131. doi: 10.1152/ajpheart.00267.2016.

Nemes O, Kovacs N, Szujo S, Bodis B, Bajnok L, Buki A, Doczi T, Czeiter E, Mezosi E. Can early clinical parameters predict post-traumatic pituitary dysfunction in severe traumatic brain injury? Acta Neurochir (Wien). 2016 Dec;158(12):2347-2353. doi: 10.1007/s00701-016-2995-x.

Buki A, Kovacs N, Czeiter E, Schmid K, Berger RP, Kobeissy F, Italiano D, Hayes RL, Tortella FC, Mezosi E, Schwarcz A, Toth A, Nemes O, Mondello S. Minor and repetitive head injury. Adv Tech Stand Neurosurg. 2015;42:147-92. doi: 10.1007/978-3-319-09066-5_8.

Bukovics P, Czeiter E, Amrein K, Kovacs N, Pal J, Tamas A, Bagoly T, Helyes Z, Buki A, Reglodi D. Changes of PACAP level in cerebrospinal fluid and plasma of patients with severe traumatic brain injury. Peptides. 2014 Oct;60:18-22. doi: 10.1016/j.peptides.2014.07.001.

 
  1. CENTER-TBI “Collaborative European NeuroTrauma Effectiveness Research in TBI” - The European Union FP 7th Framework program (grant 602150); 2013.
  2. Hungarian Brain Research Program 2.0 – Grant No. 2017-1.2.1-NKP-2017-00002; 2017.
  3. GINOP-2.3.2-15-2016-00048 “Életet veSzélyezTető Akut megbetegedések súlYossági és hALálozási mutatóInak jaVítása transzlációs orvostudományi mEgközelítésben – STAY ALIVE”; 2017.
  4. EFOP-3.6.2.-16-2017-00008 “The role of neuro-inflammation in neurodegeneration: from molecules to clinics”; 2017.
  5. OTKA FK123798 – Mechanisms of cerebrovascular dysfunction following traumatic brain injury – Tóth P.; 2017.
  6. GINOP-2.2.1-15-2017-00067 – „Networked Analytical Opportunities and Data Utilization in Healthcare”; 2017.
  7. Higher Education Institutional Excellence Programme 2.0 – 20765-3/2018/FEKUTSTRAT; 2018.
  8. OTKA K129247 – Synergistic interactions between nicotinic and NMDA receptor agents in preclinical animal models of neurocognitive disorders: neuroimaging, electrophysiology and behavior – Hernádi I.; 2018.
  1. Physiologically monitorized experimental traumatic brain injury of rats and/or mice by impact acceleration or fluid percussion head injury models.
  2. Protein biomarker measurement from animal/human biofluids.
  3. Tissue preparation for silver staining and/or immunohistochemistry investigations.
  1. Marmarou-type impact acceleration TBI model
  2. Fluid percussion head injury model
  3. Serum biobank (3 pieces of Telstar Igloo U830 830 liters capacity ultralow freezer)
  4. BMG Labtech CLARIOstar multi-mode microplate reader

Europe Biobank Week 2018

Europe Biobank Week 2019

CONTACT
Prof. Dr. András Büki
Research Group Leader