Type of shared service:
Instrument
Core Facility:
The digital flow cytometer contains 3 lasers (violet, blue and red) and 8 fluorescent channels.
Optical system
- Detection of 8 FL-parameters
- Detection of forward scatter light (FSC) with photo diode
- Direction of side scatter light (SSC) and fluorescence signal towards PMTs
Blue laser detection (488 nm)
- 502 LP, 530/30 nm FITC, Alexa Fluor 488, CFSE
- 556 LP, 585/42 nm PE, PI
- 655 LP, 670 LP nm PerCP, PerCP-Cy5.5, 7AAD, PI
- 735 LP, 780/60 nm PE-Cy7, PE-Vio780
Red laser detection (633 nm)
- 660/20 nm APC, Cy5, Alexa Fluor 647
- 735 LP, 780/60 nm APC-Cy7, APC-H7, APC-Vio780
Violet laser detection (405 nm)
- 450/50 nm PacificBlue, BV421, Alexa Flour405, BV421, eF450, Horizon V450, VioBlue
- 502 LP, 510/50 nm AmCyan, BV510, PacificOrange, Horizon V500, Q-Dot525, VioGreen
Sample Input Sizes
- Microtubes, 12x75 mm, and 15mL
The flow cytometer can be used to analyze all kinds of cell subsets in cell suspension, their DNA content, their cytokine and chemokine production.
High Throughput Sampler (HTS) option.
Registered users have to book an appointment in Szentagothai Research Centre website. Unregistered people should contact the device manager.
Contact person:
Bookable
Fee:
No