Type of shared service:
Instrument
Core Facility:
In a Total Internal Reflection Fluorescence Microscope (TIRFM), the excitation beam suffers total reflection at the cover plate-sample interface generating evanescent wave that penetrates into the environment of the cover. The penetration depth of the evanescent wave is 50–100 nm axially into the cell. Accordingly, the evanescent wave makes the fluorescent markers fluoresce in this relatively thin layer. Importantly, TIRFM provides a good signal/noise ratio with good resolution in membrane region. Accordingly, the TIRFM is excellent tool for single molecule tracking.
Microscope: Olympus IX81S1F-ZDC2
Camera: Hamamatsu C9300
Lasers: 491nm, 640nm
Objectives: 10x, 20x, 60x, 100x
Bookable
Fee:
Yes