About us

A Szentágothai János Kutatóközpont a PTE korszerű, nemzetközi tudományszervezési és menedzsment normák szerint kialakított új intézménye, amely az élettudományi, élettelen természettudományi, valamint környezettudományi oktatás...



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Isolation and characterization of extracellular vesicles

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Extracellular vesicles (EVs) are membrane-delimited particles released from all type of cells into the extracellular space. According to their size and biogenesis EVs can be differentiated into three subgroups such as exosomes, microvesicles and apoptotic bodies. These vesicles can be isolated from many body fluids (blood, urine, breast milk etc.) or from cell culture supernatant. Apoptotic bodies and microvesicles can be elutriated using high-speed centrifugation, but exosomes require PEG-based isolation method.

We are able to isolate exosomes from the following fluids:

  • blood serum
  • blood plasma
  • urine
  • cell culture supernatant
  • other body fluid (e.g. milk)

In our laboratory, it is possible to characterize the isolated vesicles (based on size and quantity), which is performed with the Malvern NanoSight NS300 device using nanoparticle tracking analysis (NTA).

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Based on Cooperation Agreement