In the structure of the Stimulated Emission Depletion (STED) microscope the illumination is supplemented by a so-called STED beam. This STED beam is concentric with the excitation beam with a donut-like intensity profile. The STED beam resets the excited fluorophores by forced emission, depending on its intensity, thus reducing the spot size where effective excitation is achieved. This method provides 50 nm resolution in x-y plane.
System: Abberiror Intruments expert line / FLIM,
Frame: Nikon Eclipse Ti2
Excitation wavelengths: 488 nm, 561 nm, 640 nm
STED laser: 775 nm
Objective: SR HP Apo F 100xAC OIL
Detector: HybridGasp, APD