About us

A Szentágothai János Kutatóközpont a PTE korszerű, nemzetközi tudományszervezési és menedzsment normák szerint kialakított új intézménye, amely az élettudományi, élettelen természettudományi, valamint környezettudományi oktatás...

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Imaging

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  • Publications

The Imaging Core Facility provides expertise and instrumentation for the performance of routine histological procedures and standard light- and laser microscopy. The Core Facility includes a fluorescence spectroscopy laboratory, supervised by Prof. János Erostyák, which allows for the spectrofluorometric characterization of inorganic and biological samples.

Services

Nikon Inverted Microscope Eclipse Ti-U

The Eclipse Ti-U uses Nikon’s world-famous CFI60 optical system and a wide choice of objectives for bright field, dark field, fluorescence, phase contrast, DIC and modulation contrast microscopy techniques.



MicroBrightField Neurolucida system

The most advanced system for neuron tracing and reconstruction, quantitative neuron analysis, and 3D brain mapping.

Neurolucida is a microscopy system specifically designed for performing accurate neuron reconstructions directly from histological specimens. It is capable of over 500 quantitative morphometric analyses, including:

The number of dendrites, axons, nodes, synapses, and spines

The length, width, and volume of dendrites and axons

The area and volume of somas

The complexity and extension of neurons  



MicroBrightField Stereo Investigator system

A Stereo Investigator system provides you with accurate, unbiased estimates of the number, length, area, and volume of cells or biological structures in a tissue specimen. It is a key research tool that has led to advances in numerous areas of neuroscience including neurodegenerative diseases, neuropathy, memory, and behavior, as well as other research fields including pulmonary research, spinal cord research, and toxicology.



Fluoreszcencia Spektroszkópia Laboratórium

Széles biológiai és biomedikális területen alkalmazható fluoreszcenciás anyagok teljes körű spektrofluorometriás jellemzése.

Lumineszcens kvantum dot-ok (QD), Szén-nanocsövek (Single Wall Carbon Nanotube, SWCN), Lanthanida komplexek, Fehérjék spektroszkópiai jellemzése.

Összetett minták numerikus színképi analízise, adatbányászat. Komplex minták gyors sorozatelemzéséhez mérési módszer kidolgozása MISF alapokon (Matrix Isopotential Synchronous Fluorimetry).

Spektrális mérések anyagvizsgálathoz, fényforrások bevizsgálásához, összehasonlító elemzéséhez a 200 – 1600 nm tartományon.

(lab-mol biofizika kcs), Erostyíák

Mért jellemzők:

  • Gerjesztési színképek
  • Emissziós színképek
  • Szinkrongerjesztési színképek
  • Gerjesztési-emissziós mátrixok (EEM)
  • Lumineszcencia lecsengési görbék
  • Idő-emissziós mátrixok (TEM)
  • Anizotrópia színképek
  • Anizotrópia lecsengési görbék
  • Kvantumhatásfok

 

Eszközök:

  • HORIBA Jobin-Yvon Nanolog spektrofluorométer 200-1600 nm (UV-VIS-NIR) spektrális tartományon és 50 ps – 100 ns időtartományon történő mérésekhez;

 

http://www.nyitottlabor.hu/profilok/15/15



Equipments

Leica VT1200 S Fully automated vibrating blade microtome

Leica VT1200 S Fully automated vibrating blade microtome

The fully automated Leica VT1200 S vibrating blade microtome is designed to meet the highest sectioning demands for cutting fresh and fixed tissue in Neuroscience.



Leica CM1860 Cryostat

Leica CM1860 - Cryostat for Standard Applications in the Clinical Histopathology Laboratory

The Leica CM1860 is your perfect assistant for high-quality, fast and safe sectioning. This high-throughput cryostat delivers diagnostic confidence by reliably producing quality sections to help provide an accurate diagnosis - even with the most complicated tissue types.



Krisztina Garai
assistant
gakr@gamma.ttk.pte.hu
29168

2024

Pubblications

Kovács P., Kitka T., Bali ZK., Nagy, LV., Bodó, A., Kovács-Öller, T., Péterfi, Z., Hernádi, I. (2024) Chemogenetic inhibition of the lateral hypothalamus effectively reduces food intake in rats in a translational proof-of-concept study. Scientific Reports 14: 11402.

Halász, H., Tárnai, V., Matkó, J., Nyitrai, M., Szabó-Meleg, E. (2024) Cooperation of Various Cytoskeletal Components Orchestrates Intercellular Spread of Mitochondria between B-Lymphoma Cells through Tunnelling Nanotubes. Cells 13: 607.

Czárán, D., Sasvári, P., Horváth, ÁI., Ella, K., Sűdy, ÁR., Borbély, É., Rusznák, K., Czéh, B., Mócsai, A., Helyes, Z., Csépányi-Kömi, R. (2023) Lacking ARHGAP25 mitigates the symptoms of autoantibody-induced arthritis in mice. Frontiers in Immunology 14: 1182278.

Madarász, T., Brunner, B., Halász, H., Telek, E., Matkó, J., Nyitrai, M., Szabó-Meleg, E. (2023) Molecular Relay Stations in Membrane Nanotubes: IRSp53 Involved in Actin-Based Force Generation. International Journal of Molecular Sciences 24: 13112.

Rusznák, K., Horváth, ÁI., Pohli-Tóth, K., Futácsi, A., Kemény, Á., Kiss, G., Helyes, Z., Czéh, B. (2022) Experimental Arthritis Inhibits Adult Hippocampal Neurogenesis in Mice. Cells 11: 791.

Csabai D, Wiborg O, Czéh B. Reduced Synapse and Axon Numbers in the Prefrontal Cortex of Rats Subjected to a Chronic Stress Model for Depression. Front Cell Neurosci. 2018 Jan 30;12:24. doi: 10.3389/fncel.2018.00024. eCollection 2018.

Czéh B, Vardya I, Varga Z, Febbraro F, Csabai D, Martis LS, Højgaard K, Henningsen K, Bouzinova EV, Miseta A, Jensen K, Wiborg O. Long-Term Stress Disrupts the Structural and Functional Integrity of GABAergic Neuronal Networks in the Medial Prefrontal Cortex of Rats. Front Cell Neurosci. 2018 Jun 20;12:148. doi: 10.3389/fncel.2018.00148. eCollection 2018.

Rusznák K, Csekő K, Varga Z, Csabai D, Bóna Á, Mayer M, Kozma Z, Helyes Z, Czéh B. Long-Term Stress and Concomitant Marijuana Smoke Exposure Affect Physiology, Behavior and Adult Hippocampal Neurogenesis. Front Pharmacol. 2018 Jul 23;9:786. doi: 10.3389/fphar.2018.00786. eCollection 2018.

2018

Pubblications

Csabai D, Wiborg O, Czéh B. Reduced Synapse and Axon Numbers in the Prefrontal Cortex of Rats Subjected to a Chronic Stress Model for Depression. Front Cell Neurosci. 2018 Jan 30;12:24. doi: 10.3389/fncel.2018.00024. eCollection 2018.

Czéh B, Vardya I, Varga Z, Febbraro F, Csabai D, Martis LS, Højgaard K, Henningsen K, Bouzinova EV, Miseta A, Jensen K, Wiborg O. Long-Term Stress Disrupts the Structural and Functional Integrity of GABAergic Neuronal Networks in the Medial Prefrontal Cortex of Rats. Front Cell Neurosci. 2018 Jun 20;12:148. doi: 10.3389/fncel.2018.00148. eCollection 2018.

Rusznák K, Csekő K, Varga Z, Csabai D, Bóna Á, Mayer M, Kozma Z, Helyes Z, Czéh B. Long-Term Stress and Concomitant Marijuana Smoke Exposure Affect Physiology, Behavior and Adult Hippocampal Neurogenesis. Front Pharmacol. 2018 Jul 23;9:786. doi: 10.3389/fphar.2018.00786. eCollection 2018.

2017

Pubblications

Csabai D, Seress L, Varga Z, Ábrahám H, Miseta A, Wiborg O, Czéh B. Electron Microscopic Analysis of Hippocampal Axo-Somatic Synapses in a Chronic Stress Model for Depression. Hippocampus. 2017 Jan;27(1):17-27. doi: 10.1002/hipo.22650. Epub 2016 Sep 15.

Varga Z, Csabai D, Miseta A, Wiborg O, Czéh B. Chronic stress affects the number of GABAergic neurons in the orbitofrontal cortex of rats. Behav Brain Res. 2017 Jan 1;316:104-114. doi: 10.1016/j.bbr.2016.08.030.

CONTACT
Dr. Boldizsár Czéh
Core Facility Leader
 +36 72 536001 /31231
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